Applied Chemical Engineering

Bibliometric analysis (VOSviewer): Analytical chemistry techniques for heavy metals and lead determination in Iraq: A review

Arwa Mahmoud Hussain

Chemistry Department, College of Science, Mustansiriyah University, Baghdad, 10045, Iraq

Ruba Fahmi Abbas

Chemistry Department, College of Science, Mustansiriyah University, Baghdad, 10045, Iraq

Shurooq Abdulfattah Hasan

Chemistry Department, College of Science, Mustansiriyah University, Baghdad, 10045, Iraq

Mohammed Jasim M. Hassan

Chemistry Department, College of Science, Mustansiriyah University, Baghdad, 10045, Iraq

DOI: https://doi.org/10.59429/ace.v8i1.5646

Keywords: VOSviewer; heavy metals; analytical chemistry; bibliometric analysis; Iraq

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Abstract

This review provides a comprehensive overview of heavy metal and lead analysis in environmental, drug, cosmetic, and food pollution samples, focusing on research conducted in Iraq. The explore of the co-occurrence of heavy metals and analytical chemistry techniques in published studies  over the last ten years was achieved by using  bibliometric analysis (VOS viewer) based on database from the Scopus.  For determining heavy metal concentrations in   different environmental samples, various analytical chemistry techniques were assesses in this review such as Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Atomic Absorption Spectrometry (AAS), and X-ray fluorescence (XRF). This study confirms the importance of development of efficient, selective, and sensitive techniques for heavy metal detection in Iraq to reduce their harmful impacts on human health and the environment.

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References

[1]. Mitra, S., Chakraborty, A. J., Tareq, A. M., Emran, T. B., Nainu, F., Khusro, A., ... & Simal-Gandara, J. (2022). Impact of heavy metals on the environment and human health: Novel therapeutic insights to counter the toxicity. Journal of King Saud University-Science, 34(3), 101865.

[2]. Upadhyay, R. (2022). Heavy metals in our ecosystem. In Heavy Metals in Plants (pp. 1-15). CRC Press.

[3]. Witkowska, D., Słowik, J., & Chilicka, K. (2021). Heavy metals and human health: Possible exposure pathways and the competition for protein binding sites. Molecules, 26(19), 6060.

[4]. Aleluia, A. C. M., de Souza Nascimento, M., dos Santos, A. M. P., dos Santos, W. N. L., Júnior, A. D. F. S., & Ferreira, S. L. C. (2023). Analytical approach of elemental impurities in pharmaceutical products: a worldwide review. Spectrochimica Acta Part B: Atomic Spectroscopy, 205, 106689.

[5]. Finotti Cordeiro, C., Lopardi Franco, L., Teixeira Carvalho, D., & Bonfilio, R. (2024). Impurities in Active Pharmaceutical Ingredients and Drug Products: A Critical Review. Critical Reviews in Analytical Chemistry, 1-21.

[6]. Aleluia, A. C. M., de Souza Nascimento, M., dos Santos, A. M. P., dos Santos, W. N. L., Júnior, A. D. F. S., & Ferreira, S. L. C. (2023). Analytical approach of elemental impurities in pharmaceutical products: a worldwide review. Spectrochimica Acta Part B: Atomic Spectroscopy, 205, 106689.

[7]. Radić, I., Runje, M., Cvetnić, M., Halužan, M., Jeličić, M. L., Miloloža, M., ... & Bolanča, T. (2021). Validation of the ICP-MS method for determination of trace elements provided by ICH Q3D guideline in fosamprenavir calcium. Kemija u industriji: Časopis kemičara i kemijskih inženjera Hrvatske, 70(11-12), 701-709.

[8]. Torres, S., Boetzel, R., Gatimu, E., Gomes, D. Z., King, F., Kocks, G., ... & Teasdale, A. (2022). ICH Q3D drug product elemental risk assessment: The use of an elemental impurities excipients database. Journal of Pharmaceutical Sciences, 111(5), 1421-1428.

[9]. Satoh, M., Motokawa, K., Uesawa, Y., Ishikawa, Y., Maeda, H., Iida, K., ... & Nishizawa, K. (2021). Study of the compatibility of oral magnesium oxide preparations sold in Japan with the ICH-Q3D guideline for elemental impurities. Drug Discoveries & Therapeutics, 15(5), 227-235.

[10]. Annar, S. (2022). The characteristics, toxicity and effects of heavy metals arsenic, mercury and cadmium: A review. Int. J. Multidiscip. Educ. amazonaws. com.

[11]. Khatun, J., Intekhab, A., & Dhak, D. (2022). Effect of uncontrolled fertilization and heavy metal toxicity associated with arsenic (As), lead (Pb) and cadmium (Cd), and possible remediation. Toxicology, 477, 153274.

[12]. Li, B., Hu, K., Lysenko, V., Khan, K. Y., Wang, Y., Jiang, Y., & Guo, Y. (2022). A scientometric analysis of agricultural pollution by using bibliometric software VoSViewer and Histcite™. Environmental Science and Pollution Research, 29(25), 37882-37893.

[13]. Hirawan, D., Oktafiani, D., Fauzan, T. A., Luckyardi, S., & Jamil, N. (2022). Research trends in farming system soil chemical: A bibliometric analysis using VOSviewer. Moroccan Journal of Chemistry, 10(3), J-Chem.

[14]. Zhang, K., Liu, F., Zhang, H., Duan, Y., Luo, J., Sun, X., ... & Li, D. (2024). Trends in phytoremediation of heavy metals-contaminated soils: a web of science and CiteSpace bibliometric analysis. Chemosphere, 352, 141293.

[15]. Benhander, G. M. (2024). Mapping Research Landscape: Bibliometric Analysis of Microextraction Techniques in Drug Analysis Using Scopus Database. Analytical and Bioanalytical Chemistry Research, 11(2), 211-225.

[16]. Saxena, K. (2024). Study of Poly (amidoamine) Based Dendrimers as Adsorbents for Heavy Metals Removal Through Bibliometric Analysis. Indonesian Journal of Limnology, 5(1), 49-63.

[17]. Shi, D., Xie, C., Wang, J., & Xiong, L. (2021). Changes in the structures and directions of heavy metal-contaminated soil remediation research from 1999 to 2020: A bibliometric & scientometric study. International Journal of Environmental Research and Public Health, 18(14), 7358.

[18]. Carbonell-Rozas, L., Lara, F. J., & García-Campaña, A. M. (2024). Analytical methods based on liquid chromatography and capillary electrophoresis to determine neonicotinoid residues in complex matrices. A comprehensive review. Critical Reviews in Analytical Chemistry, 54(7), 2554-2582.

[19]. Muhammad, N., Hussain, I., Ali, A., Noureen, L., He, Q., Subhani, Q., ... & Zhu, Y. (2024). Ion chromatography: A comprehensive review of sample preparation methods for analysis of halogens and allied nonmetals in critically challenging inorganic matrices. Journal of Chromatography A, 465311.

[20]. Lata, S., & Ansari, N. G. (2024). Analytical Techniques for Heavy Metal Analysis. In Heavy Metal Contamination in the Environment (pp. 180-202). CRC Press.

[21]. Muhammad, N., Lao, L., Intisar, A., Cui, H., & Zhu, Y. (2019). Simultaneous determination of fluoride and chloride in iron ore by steam distillation followed by ion chromatography. Chromatographia, 82(12), 1839-1844.

[22]. Muhammad, N., Zia-ul-Haq, M., Ali, A., Naeem, S., Intisar, A., Han, D., ... & Wei, B. (2021). Ion chromatography coupled with fluorescence/UV detector: A comprehensive review of its applications in pesticides and pharmaceutical drug analysis. Arabian Journal of Chemistry, 14(3), 102972.

[23]. Golgoli, T., Ghanemi, K., & Buazar, F. (2024). Cysteine-functionalized selenium nanoparticles for efficient extraction and preconcentration of cadmium, copper, lead, and zinc ions from environmental water samples. Journal of Molecular Liquids, 393, 123680.

[24]. Ding, Q., Li, C., Wang, H., Xu, C., & Kuang, H. (2021). Electrochemical detection of heavy metal ions in water. Chemical communications, 57(59), 7215-7231.

[25]. Ding, R., Cheong, Y. H., Ahamed, A., & Lisak, G. (2021). Heavy metals detection with paper-based electrochemical sensors.

[26]. Lace, A., & Cleary, J. (2021). A review of microfluidic detection strategies for heavy metals in water. Chemosensors, 9(4), 60.

[27]. D’Atri, V., Barrientos, R. C., Losacco, G. L., Rudaz, S., Delobel, A., Regalado, E. L., & Guillarme, D. (2025). Trends in Pharmaceutical Analysis: The Evolving Role of Liquid Chromatography. Analytical Chemistry.

[28]. Sharma, S., Singh, N., Ankalgi, A. D., Rana, A., & Ashawat, M. S. (2021). Modern trends in analytical techniques for method development and validation of pharmaceuticals: A review. J. Drug Deliv. Ther, 11, 121-130.

[29]. Lin, S. Y. (2021). Current and potential applications of simultaneous DSC-FTIR microspectroscopy for pharmaceutical analysis. Journal of Food and Drug Analysis, 29(2), 182.

[30]. Fahelelbom, K. M., Saleh, A., Al-Tabakha, M. M., & Ashames, A. A. (2022). Recent applications of quantitative analytical FTIR spectroscopy in pharmaceutical, biomedical, and clinical fields: A brief review. Reviews in Analytical Chemistry, 41(1), 21-33.

[31]. Buledi, J. A., Shah, Z. U. H., Mallah, A., & Solangi, A. R. (2022). Current perspective and developments in electrochemical sensors modified with nanomaterials for environmental and pharmaceutical analysis. Current Analytical Chemistry, 18(1), 102-115.

[32]. AYDOĞMUŞ, Z., & Asfoor, A. (2022). Electroanalytical methods for determination of antiviral drugs in pharmaceutical formulation and biological fluids: a review. Chinese Journal of Analytical Chemistry, 50(10), 100063.

[33]. Hudhaifa, A. H., Aljumaily, M. M., & Al-Obaidi, M. A. (2024). Mono and competitive adsorption of Zn, Cu and Mn in different calcareous soils. REVISTA DE AGRICULTURA NEOTROPICAL, 11(3), e8543-e8543.

[34]. Jarjees, F. Z., & Darwesh, D. A. (2024). Human Health Risk Assessment of Toxic Heavy Metals in Commercially Available Rice at Local Markets in Erbil, within the Kurdistan Region of Iraq. Zanco Journal of Pure and Applied Sciences, 36(3), 95-108.

[35]. Al-Darraji, J., Alshami, I., & Ankush, M. (2023). Effect of Some Heavy Metals in the Industrial Flows on Shatt al-Arab River. Egyptian Journal of Aquatic Biology and Fisheries, 27(3), 801-814.

[36]. Othman, H. Q., & Hussein, Z. A. (2025). Radiological impacts of natural radioactivity and heavy metal of building material factories in Sulaymaniyah Governorate, Iraq. International Journal of Environmental Analytical Chemistry, 1-24.

[37]. Baddi, I., Al-Mallah, A., & Omer, F. (2024). An assessment of heavy metals soil Pollution and health risk around industrial district in Qayarah area, Northern Iraq. Iraqi National Journal of Earth Science, 24(2), 72-0.

[38]. Samir, B. A., & Kosaj, A. D. (2024). International Journal of Environmental Impacts. Journal homepage: http://iieta.org/journals/ijei, 7(2), 329-338.

[39]. Hasan, A. A., Al-Alawy, I. T., & Kadhim, H. A. (2024). Water Quality Assessment and Evaluation of the Human Health Risk of Tap Water Use in the Karbala Governorate, Iraq. Radiochemistry, 66(5), 764-773.

[40]. Jaafar, B. I., & Kadhum, S. A. (2023, November). Bioaccessibility and Distribution of Heavy Metals in Particles from a Dust Storm in Iraq. In IOP Conference Series: Earth and Environmental Science (Vol. 1259, No. 1, p. 012040). IOP Publishing.

[41]. Hussein, S. M., Jazza, S. H., & Najim, S. S. (2023). Bioaccumulation of heavy metals in liver tissues of three Anas species in Al-Hawizah marshes, southern Iraq. International Journal of Aquatic Biology, 11(2), 98-103.

[42]. Ghazi, A. M., & Marhoon, K. A. (2024, July). Determination of Heavy Metals Concentration in the Blood Samples of some Farmers at Al-Diwaniyah Governorate/Iraq. In IOP Conference Series: Earth and Environmental Science (Vol. 1371, No. 2, p. 022023). IOP Publishing.

[43]. Hammash, S. M., & Abed, M. F. (2022). Environmental Evaluation of the Surface Water, Ground Water and Wastewater using Pollution Indices at the Landfill Site, Southern Baiji, Salah Al-Din Northern iraq. The Iraqi Geological Journal, 105-112.

[44]. Issa, H. M., & Alshatteri, A. H. (2021). Impacts of wastewater discharge from Kalar city on Diyala-Sirwan river water quality, Iraq: pollution evaluation, health risks of heavy metals contamination. Applied Water Science, 11(4), 73.

[45]. Hamdy, S. M. A., Al Obiady, A. H. M. J., & Al Mashhady, A. A. M. (2015). Effects of lead pollution on soil and plants around the powered generators. Iranian Journal of Science, 39(3), 341-343.

[46]. Basha, N. T., & Issa, M. J. (2025). Determine the Pollution Indices of the Soil Near the Najaf Refinery in the Middle of Iraq. Iraqi Journal of Science, 219-229.

[47]. Al-Zubaydi, J. H., Al-Sultani, N. K., & Al-Amar, H. A. (2024, July). Hydrological Study of the Great Musaiyab Project Babylon Governorate in Middle of Iraq. In IOP Conference Series: Earth and Environmental Science (Vol. 1371, No. 8, p. 082005). IOP Publishing.

[48]. Al-Obeidi, L. A. A., & Al-Hamadawi, H. A. (2024). Assessment of heavy metals on some tissues of Marbled Teal, Marmaronetta Angustirostris in Al-Dalmaj Marsh, Iraq. International Journal of Aquatic Biology, 12(1), 25-32.

[49]. Ghaidan, H. Q., & Al-Dhamin, A. S. A. (2024). Detection of Some Heavy Metals (Mercury and Lead) Pollutants and their Association with Type 2 Diabetes Mellitus (T2DM) in Baghdad, Iraq. Iraqi Journal of Science, 6353-6370.

[50]. salman obaid AL-khazraji, S., & Al-Fatlawy, H. J. J. (2024). The Concentration of Some Heavy Metals in Phragmites Australis and Ceratophyllum. Demersum Plants in the Euphrates River, Iraq. Pak. J. Life Soc. Sci., 22, 2881-2903.

[51]. Salih, Z. R., Othman, B. A., & Aweez, S. J. (2024). Assessment of heavy metals in rainfall as an indicator of air pollution from Erbil Steel Factory in Iraq. Environmental Monitoring and Assessment, 196(3), 319.

[52]. Khalefah, A. R. M., Omran, I. I., & Al Waily, M. J. (2024). Environmental Impact of Petroleum Refinery Effluent on Groundwater Pollution: A Case Study of Maysan Refinery, Iraq. Salud, Ciencia y Tecnología-Serie de Conferencias, (3), 844.

[53]. Al-Fartusie, F. S., Mohammed, M. A., Thani, M. Z., Kader, S., & Khadim, R. M. (2024). Evaluation of Heavy Metal and Specific Trace Elements Levels Among Fast-Food Workers and Their Susceptibility to Atherosclerosis. Biological Trace Element Research, 1-10.

[54]. Ibraheem, A. G., Jawad, M. M. H. M., & Aqeel Lami, A. M. A. (2024). Evaluation of Lead, Copper and Iron Concentrations in Cyprus papyrus and Rivers in Iraq. Asian Journal of Water, Environment and Pollution, 21(5), 49-55.

[55]. Qadr, H. M., Ibrahim, M. H., & Hassan, H. Y. (2025). Determination of potentially toxic heavy metals in milk powder marketed in Kurdistan of Iraq. Journal of Trace Elements and Minerals, 100219.

[56]. Almola, M. A., Al-Ahmady, K. K., & Mhemid, R. K. S. (2024). An Investigation of Microplastic Occurrence and Heavy Metals Concentrations in Street Dust on the Left Side of Mosul City, Iraq. Journal of Ecological Engineering, 25(9).

[57]. Al-Lami, A. M. A., Abbas, Z. R., & Alshammary, A. G. (2020). Study of water pollution problem in Basra city of Iraq: a case study.

[58]. Al-Mayyahi, S. O. M., & Al-Zamili, H. A. A. (2019, July). Assessment of some heavy metals contamination in sediments of Tigris River in Kut City, Wasit Governorate, Iraq. In Journal of Physics: Conference Series (Vol. 1234, No. 1, p. 012076). IOP Publishing.

[59]. Znad, S. R., & Al-Sinjary, M. N. (2020). Assessment of heavy metal pollution of industrial zones in mosul city. Plant Archives, 20(2), 256-263.

[60]. Saeed, A. M., Hamzah, M. J., & Ahmed, N. Q. (2018). Quantitative assay of aspirin and (salicylic acid and heavy metals as impuraties) in iraqi’s market aspirin tablets using different analytical methods. Int J App Pharm, 10(5), 167-172.

[61]. Hussain, H. A., Abdullah, S. A., & Al Maliki, A. A. (2023, July). Predicting of heavy metals in some areas of Iraq using spectral analysis techniques. In IOP Conference Series: Earth and Environmental Science (Vol. 1202, No. 1, p. 012009). IOP Publishing.

[62]. Mahdi, H. J. (2022). Evaluation of the Pharmaceutical and Microbial Safety, as well as the Reliability, of Some Natural Pharmaceutical Products Available in Iraq. Archives of Razi Institute, 77(4), 1465-1471.

[63]. Abu-ALmaaly, R. A., Karm, I. F. A., & Alsaffar, N. M. A. (2018). Nutrient value and contamination by arsenic, mercury and cadmium in rice types available in local markets. Journal of Pharmaceutical Sciences and Research, 10(7), 1761-1764.

[64]. Lawi, D. J., Abdulwhaab, W. S., & Abojassim, A. A. (2023). Health risk study of heavy metals from consumption of drugs (solid and liquid) samples derived from medicinal plants in Iraq. Biological Trace Element Research, 201(7), 3528-3540.

[65]. Khaleel, L. R., Al-Hermizy, S. M., & Abbas, M. N. (2022). Statistical Indicators for Evaluating the Effect of Heavy Metals on Samaraa Drug Industry Water Exposed to the Sun and Freezing. Tropical Journal of Natural Product Research, 6(12).

[66]. Alsamaraay, R. T. A., Jabbar, R. A. A., & Alshawany, T. M. (2021, May). Quality study of the piped water of state company for drugs industry of Samarra and matching with the Iraqi and international drinking specification. In Journal of Physics: Conference Series (Vol. 1879, No. 2, p. 022032). IOP Publishing.

[67]. Al-Dabbas, M. A., Mahdi, K. H., Al-Khafaji, R., & Obayes, K. H. (2018, May). Heavy metals characteristics of settled particles of streets dust from Diwaniyah City-Qadisiyah Governorate-Southern Iraq. In Journal of Physics: Conference Series (Vol. 1003, No. 1, p. 012023). IOP Publishing.

[68]. Awadh, S. M. (2015). Cd, Ni, and Pb distribution and pollution assessment in roadside dust from Baghdad City and Western Iraqi Desert. Arab J Geosci 8: 315–323.

[69]. Dalaram, I. S., Nabil, R. H., & Dina, A. S. (2016). THE RELATIONSHIP OF HEAVY METALS CONTENTS IN SOILS TO THEIR CONTENT IN LEGUME SEEDS USED IN FAMOUS TRADITIONAL FOOD IN KURDISTAN REGION-IRAQ. Slovak Journal of Food Sciences/Potravinarstvo, 10(1).

[70]. Sayyadi, A., & Ioannidu, S. (2015). Determination of lead content in the most popular brands of lipsticks in Sulaimani, Iraq. Journal of the Indian Chemical Society, 92(11), 1625-1628.

[71]. Sadoon, M. A., & Ali, S. A. (2019). ASSESSMENT OF HEAVY METALS LIKE LEAD, CADMIUM AND COPPER IN MEDICINAL PLANTS COLLECTED FROM MARKET OF NASERIYA CITY, IRAQ. Plant Archives, 19(2), 2357-2358.

[72]. Abdulnabi, Z. A., Altememi, M. K., Hassan, W. F., Al-Khuzaie, D. K. K., & Saleh, S. M. (2019). Assessing of some toxic heavy metals levels and using geo accumulation index in sediment of Shatt Al-Arab and the Iraqi Marine region. Baghdad Science Journal, 16(2), 323-331.

[73]. Ali, N. A., & Abdullah, E. J. (2024). Accumulation, Bioavailability, and Health Risk of Heavy Metals in Some Plants Obtained from Abu-Ghraib Land, Baghdad, Iraq. The Iraqi Geological Journal, 187-200.

[74]. AL-Shammary, A. G., & Al-Jomaily, M. (2024). Evaluation of environmental and pollution indices of heavy metals of dust samples in Baiji and Salah Al-Din General Hospitals in Salah Al-Din Governorate/Iraq. Iraqi National Journal of Earth Science, 24(1), 17-0.

[75]. Jassim, H. H., Salman, I. M., & Al-Khafaji, R. M. N. (2021). Sedimentological and heavy metals characteristics of streets dust in some areas east of Baghdad for 2020. The Iraqi Geological Journal, 117-125.

[76]. Kareem, D. O., Ibrahim, A. A., & Ibrahiem, O. S. (2020). Heavy metal and radon gas concentration levels in Khasa River in Kirkuk City (NE Iraq) and the associated health effects. Arabian Journal of Geosciences, 13(19), 1023.

[77]. Joda, B. A., Alheloo, H. S., Al-Mankosh, H. J., & Maitham, S. A. (2019, August). Determination of heavy metals arsenic, cadmium and lead in water, sediments and fish from Al Delmaj Marshes-Iraq. In AIP Conference Proceedings (Vol. 2144, No. 1). AIP Publishing.

[78]. Khalaf, E. A., Abduljaleel, S. A., & Al-Jassani, H. M. (2021). Appraisal of trace elements and heavy metals levels in breast cancer patients of Basrah Province. Toxicology International, 28(2), 8-14.