Applied Chemical Engineering

Application of central composite design and desirability function in the optimization of spectrophotometric method for quantification of fast green FCF using a green microextraction method

Zianab Tariq

Department of Chemistry, College of Sciences, University of Al-Qadisiyah, Dewanyia, 58002, Iraq

Alaa S. Alwan

Department of Chemistry, College of Sciences, University of Al-Qadisiyah, Dewanyia, 58002, Iraq

Layth S. Jasim

Department of Chemistry, College of Education, University of Al-Qadisiyah, Diwaniyah, 58002, Iraq

DOI: https://doi.org/10.59429/ace.v8i2.5603

Keywords: SHS-LPME: switchable hydrophilicity solvent liquid phase microextraction, FG: Fast green FCF and df: degree of freedom

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Abstract

The aim of this article is to develop a "switchable hydrophilicity solvent liquid phase microextraction" (SHS-LPME) for the effectual extraction of fast green FCF. Three "switchable hydrophilicity solvents" (SHSs) were practiced for the extraction of fast green FCF. The attained extract phase afterward phase separation was evaluated by UV-VIS spectrophotometry. The extraction parameters such as, SHS volume, HNO3 volume and NaOH volume, were enhanced using central composite design and desirability function. Under optimized conditions, the linear range 0.50-5.00 µg/ mL with R2 = 0.9886, limit of detection 0.341 µg/ mL, limit of quantitation 1.026 µg/ mL. The method showed a relative standard deviation (RSD) of 1.16% for 7 replicate measurements. Preconcentration and Enrichment factors were determined to be 20 and 35 respectively, indicating the method’s efficiency in enhancing fast green detection. The proposed method was applied in real samples successfully.

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References

[1]. Ntrallou, K., H. Gika, and E. Tsochatzis, Analytical and sample preparation techniques for the determination of food colorants in food matrices. Foods, 2020. 9(1): p. 58.

[2]. Rodriguez-Amaya, D.B., Natural food pigments and colorants. Current Opinion in Food Science, 2016. 7: p. 20-26.

[3]. Martins, N., et al., Food colorants: Challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices. Trends in food science & technology, 2016. 52: p. 1-15.

[4]. Abdulhusain, Z.H., L.S. Jasim, and M. Batool, Azur C Dye Removal using GO/P (CMC-Co-Am) Nanocomposite: Adsorption and Kinetic Studies. Journal of Nanostructures, 2024. 14(4): p. 1225-1238.

[5]. Alshamusi, Q.K.M., et al., Efficiency of Chitosan-Grafted Poly (Carboxymethyl Cellulose-Co-Acrylamide) Nano Hydrogel for Cadmium (II) Removal: Batch Adsorption Study. Journal of Nanostructures, 2024. 14(4): p. 1122-1133.

[6]. Alzayd, A.A.M., et al., Isotherm and Thermodynamic Analysis of Azur C Dye Adsorption on GO/P (CMC-Co-Am) Nanocomposite. Journal of Nanostructures, 2024. 14(3): p. 845-856.

[7]. Batool, M., et al., Exploring the usability of Cedrus deodara sawdust for decontamination of wastewater containing crystal violet dye. Desalination and water treatment, 2021. 224: p. 433-448.

[8]. Majeed, H.J., et al., Synthesis and application of novel sodium carboxy methyl cellulose-g-poly acrylic acid carbon dots hydrogel nanocomposite (NaCMC-g-PAAc/CDs) for adsorptive removal of malachite green dye. Desalination and Water Treatment, 2024: p. 100822.

[9]. Mannan, H.A., et al., Mesoporous activated TiO2/based biochar synthesized from fish scales as a proficient adsorbent for deracination of heavy metals from industrial efflux. Journal of Dispersion Science and Technology, 2022: p. 1-13.

[10]. Saadallah, K., et al., Potential of the Algerian pine tree bark for the adsorptive removal of methylene blue dye: Kinetics, isotherm and mechanism study. Journal of Dispersion Science and Technology: p. 1-19.

[11]. Tikhomirova, T.I., G.R. Ramazanova, and V.V. Apyari, Effect of nature and structure of synthetic anionic food dyes on their sorption onto different sorbents: Peculiarities and prospects. Microchemical Journal, 2018. 143: p. 305-311.

[12]. Arshad, R., T. Javed, and A. Thumma, Exploring the efficiency of sodium alginate beads and Cedrus deodara sawdust for adsorptive removal of crystal violet dye. Journal of Dispersion Science and Technology, 2023: p. 1-14.

[13]. Javed, T., et al., Batch adsorption study of Congo Red dye using unmodified Azadirachta indica leaves: isotherms and kinetics. Water Practice & Technology, 2024: p. wpt2024020.

[14]. Rehman, H., et al., Potential of easily available low-cost raw cotton for the elimination of methylene blue dye from polluted water. Desalination and Water Treatment, 2024. 318: p. 100319.

[15]. Bukhari, A., T. Javed, and M.N. Haider, Adsorptive exclusion of crystal violet dye from wastewater by using fish scales as an adsorbent. J. Dispers. Sci. Technol., 2022: p. 1-12.

[16]. Imran, M.S., et al., Sequestration of crystal violet dye from wastewater using low-cost coconut husk as a potential adsorbent. Water Science and Technology, 2022. 85(8): p. 2295-2317.

[17]. Urooj, H., et al., Adsorption of crystal violet dye from wastewater on Phyllanthus emblica fruit (PEF) powder: kinetic and thermodynamic. International Journal of Environmental Analytical Chemistry, 2023: p. 1-26.

[18]. Oplatowska-Stachowiak, M. and C.T. Elliott, Food colors: Existing and emerging food safety concerns. Critical reviews in food science and nutrition, 2017. 57(3): p. 524-548.

[19]. Zhu, Y., Recent advances in detection Technologies of synthetic colorants in foods. Hans Journal of Food and Nutrition Science, 2013. 2(3): p. 29-37.

[20]. Ali, M.A. and S. Bashier, Effect of fast green dye on some biophysical properties of thymocytes and splenocytes of albino mice. Food additives and contaminants, 2006. 23(05): p. 452-461.

[21]. Mittal, A., D. Kaur, and J. Mittal, Batch and bulk removal of a triarylmethane dye, Fast Green FCF, from wastewater by adsorption over waste materials. Journal of Hazardous Materials, 2009. 163(2-3): p. 568-577.

[22]. Oymak, T. and E. Dural, Determination of sunset yellow, allura red, and fast green using a novel magnetic nanoadsorbent modified with Elaeagnus angustifolia based on magnetic solid-phase extraction by HPLC. Brazilian Journal of Pharmaceutical Sciences, 2022. 58: p. e20884.

[23]. Karami, F. and A. Shokrollahi, Simultaneous Preconcentration of Fast Green FCF and Rhodamine B Using Deep Eutectic Solvent and Determination via High‐Performance Thin Layer Chromatography. Journal of Analytical Methods in Chemistry, 2023. 2023(1): p. 8797355.

[24]. Dalavi, D.K., et al., TNPs as a novel fluorescent sensor for the selective recognition of fast green FCF: a spectrofluorimetric approach. RSC Advances, 2015. 5(85): p. 69371-69377.

[25]. Shah, A., A novel electrochemical nanosensor for the simultaneous sensing of two toxic food dyes. ACS omega, 2020. 5(11): p. 6187-6193.

[26]. Lyskovtseva, K., et al., Extraction and determination of synthetic food dyes in an aqueous biphasic system based on tetrabutylammonium bromide. Journal of Analytical Chemistry, 2022. 77(10): p. 1236-1246.

[27]. Abbas, R.F., M.J.M. Hassan, and A.M. Rheima, Magnetic solid-phase extraction of Fast Green dye based on magnetic nanoparticles (GO/Co3O4/Fe3O4) prepared by photo-irradiation method. Green Analytical Chemistry, 2023. 7: p. 100086.

[28]. Abbas, R.F., M.J.M. Hassan, and A.M. Rheima, Adsorption of fast green dye onto Fe3O4 MNPs and GO/Fe3O4 MNPs synthesized by photo-irradiation method: Isotherms, thermodynamics, kinetics, and reuse studies. Sustainable Chemistry for the Environment, 2024. 6: p. 100104.

[29]. Smirnova, S.V., K.A. Lyskovtseva, and I.V. Pletnev, Extraction and determination of synthetic food dyes using tetraalkylammonium based liquid-liquid extraction. Microchemical Journal, 2021. 162: p. 105833.

[30]. Abdi, S. and M. Nasiri, Removal of Fast Green FCF dye from aqueous solutions using Flower Gel as a low-cost adsorbent. Water Science and Technology, 2018. 77(5): p. 1213-1221.

[31]. Elik, A., et al., Development of an orbital shaker-assisted fatty acid-based switchable solvent microextraction procedure for rapid and green extraction of amoxicillin from complex matrices: Central composite design. Food Chemistry, 2024: p. 139785.

[32]. Al-Nidawi, M., et al., Switchable-hydrophilicity solvent liquid-liquid microextraction prior to spectrophotometric determination of Sudan I dye in spices. Analytical Letters, 2024. 57(2): p. 202-212.

[33]. 33. Elik, A. and N. Altunay, Optimization of vortex-assisted switchable hydrophilicity solvent liquid phase microextraction for the selective extraction of vanillin in different matrices prior to spectrophotometric analysis. Food Chemistry, 2023. 399: p. 133929.

[34]. 34. Erbas, Z. and M. Soylak, Determination of rhodamine B in water and cosmetics by switchable solvent-based liquid phase microextraction with spectrophotometric determination. Instrumentation Science & Technology, 2023. 51(3): p. 290-302.

[35]. Altunay, N., Chemometric design-based optimization of a green, selective and inexpensive switchable hydrophilicity solvent-based liquid phase microextraction procedure for pre-concentration and extraction of sulfadiazine in milk, honey and water samples. Food Chemistry, 2022. 394: p. 133540.

[36]. Behpour, M., et al., Combination of gel-electromembrane extraction with switchable hydrophilicity solvent-based homogeneous liquid-liquid microextraction followed by gas chromatography for the extraction and determination of antidepressants in human serum, breast milk and wastewater. Journal of Chromatography A, 2020. 1621: p. 461041.

[37]. Hassan, M., et al., Switchable-hydrophilicity solvent liquid-liquid microextraction for sample cleanup prior to dispersive magnetic solid-phase microextraction for spectrophotometric determination of quercetin in food samples. Sustainable Chemistry and Pharmacy, 2021. 22: p. 100480.

[38]. Nasser G , Solvent Microextraction−Flame Atomic Absorption Spectrometry (SME−FAAS) for Determination of Ultratrace Amounts of Cadmium in Meat and Fish Samples., J. Agricultural and Food Chemistry 2009, 57, 3, 1099-1104

[39]. Nasser, G., Mansour, A. C., Vatankhahan, E.,Amir, H. A,.Suspended droplet solvent microextraction-flame atomic absorption spectrometry (SDSME-FAAS) determination of trace amounts of copper in river and sea water samples. Journal of Analytical Chemistry,2014. 69: p. 1061–1065.

[40]. Nasser, G.,Sahar, F .,Mansour, A C., Ghadamali, B ., Optimization of modified dispersive liquid–liquid microextraction coupled with high-performance liquid chromatography for the simultaneous preconcentration and determination of nitrazepam and midazolam drugs: An experimental design. J.Separation Science 2015, 38(10)

[41]. Moghaddam, S.S., M.A. Moghaddam, and M. Arami, Coagulation/flocculation process for dye removal using sludge from water treatment plant: optimization through response surface methodology. Journal of hazardous materials, 2010. 175(1-3): p. 651-657.