Applied Chemical Engineering

Optimization of grinding and distillation parameters affecting yield and composition of essential oils from the hybrid Eucalyptus grandis × E. camaldulensis (clone 2414)

Safaa Abid

Laboratory of Plant Chemistry, Organic and Bioorganic Synthesis, Faculty of Sciences. Mohammed V University in Rabat, 4 Avenue Ibn Battouta B.P. 1014 RP. Morocco

Badr Satrani

Center for Innovation, Research and Training, Water and Forests National Agency, (CIRF, ANEF), BP 763, 10080, Rabat, Morocco

Magri Najib

Center for Innovation, Research and Training, Water and Forests National Agency, (CIRF, ANEF), BP 763, 10080, Rabat, Morocco

Mohamed Ouajdi

Center for Innovation, Research and Training, Water and Forests National Agency, (CIRF, ANEF), BP 763, 10080, Rabat, Morocco

Ayoub Souileh

Center for Innovation, Research and Training, Water and Forests National Agency, (CIRF, ANEF), BP 763, 10080, Rabat, Morocco

Achraf Mabrouk

Center for Innovation, Research and Training, Water and Forests National Agency, (CIRF, ANEF), BP 763, 10080, Rabat, Morocco

Farah Aabouch

Plant animal production and agro-industry laboratory, Biology department, Ibn Tofail university, Kenitra, 14000, Morocco

Badr Eddine Kartah

Laboratory of Plant Chemistry, Organic and Bioorganic Synthesis, Faculty of Sciences. Mohammed V University in Rabat, 4 Avenue Ibn Battouta B.P. 1014 RP. Morocco

DOI: https://doi.org/10.59429/ace.v8i4.5775

Keywords: eucalyptus camaldulensis; eucalyptus grandis; hybrid clone 2414; grinding; distillation time; essential oils; GC–MS; yield; composition

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Abstract

This study examines how grinding and distillation duration affect the yield and composition of essential oils (EOs) from the natural hybrid Eucalyptus grandis × E. camaldulensis (clone 2414) and its parental species. Hydrodistillation was performed for 2–4 h on ground and whole leaves. Grinding increased yield by 20–25 %, and the optimum extraction time was 3 h. Yields ranged from 1.5 % (E. grandis) to 2.3 % (E. camaldulensis), with 1,8-cineole (42–61 %) as the main constituent. The hybrid exhibited a stable and distinctive chemical profile rich in oxygenated monoterpenes, demonstrating its industrial potential.

Leaves were subjected to hydrodistillation using a Clevenger type apparatus under two conditions: ground versus whole leaves, and varying distillation times (2, 3, and 4 hours). EO yields were calculated relative to dry leaf mass, and the chemical profile was determined by gas chromatography–mass spectrometry (GC–MS).

Results revealed that grinding significantly enhanced oil recovery, with ground leaves yielding up to 20–25% more oil compared to whole leaves. Distillation time strongly influenced EO output, with an optimal recovery observed at 3 hours; beyond this, additional distillation produced negligible increases. Overall yields ranged from 1.5% (E. grandis) to 2.1% (E. camaldulensis), while clone 2414 consistently displayed intermediate productivity (≈1.8%) that improved under optimized grinding and distillation conditions. GC–MS analysis confirmed 1,8 cineole as the dominant constituent (42–61%), accompanied by α-pinene, p-cymene, and limonene in variable proportions. Notably, the hybrid exhibited a distinctive chemical fingerprint enriched in oxygenated monoterpenes compared with its parents.

These findings demonstrate that essential oil yield and composition are strongly influenced by both genetic background and processing variables. Clone 2414 consistently delivered stable and enhanced oil profiles under optimized conditions, highlighting its value as a versatile resource for industrial essential oil production.

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References

[1]. Tine Y, Diallo A, Ndoye I, Gaye C, Ndiaye B, Diop A, et al. Chemical Variability and Antibacterial Activity of <i>Eucalyptus camaldulensis</i> Essential Oils from Senegal. IJOC 2022;12:173‑80.

[2]. Hassani Moghaddam E, Karamian R, Shaaban M, Mohammadian A. Identification and Comparison of Some Essential Oils Components in Seven Eucalyptus Species Cultivated in Khoramabad. JMPB [Internet] 2020 [cité 2025 sept 18];0. Available from: https://doi.org/10.22092/jmpb.2020.121751

[3]. Lu FL, Chen YY, Wei JH, Huang YL, Li DP, Liu ZY. Chemical Characterization of the Essential Oils of Eucalyptus.grandis×Eucalyptus.urophylla Hybrids and Six Pure Eucalyptus species Grown in Guangxi (China). AMR 2014;1033‑1034:200‑8.

[4]. Bibow A, Oleszek W. Essential Oils as Potential Natural Antioxidants, Antimicrobial, and Antifungal Agents in Active Food Packaging. Antibiotics 2024;13:1168.

[5]. Elaissi A, Chraif I, Bannour F, Farhat F, Salah MB, Chemli R, et al. Contribution to the Qualitative and Quantitative Study of Seven Eucalyptus Species Essential Oil Harvested of Hajeb’s Layoun Arboreta (Tunisia). Journal of Essential Oil Bearing Plants 2007;10:15‑25.

[6]. Taşdemir C. Eucalyptus camaldulensis ve E.grandis ile melezlerinin soğuğa ve okaliptus gal arısına dayanıklılığının araştırılması. Ormancılık Araştırma Dergisi 2025;12:13‑31.

[7]. Yanico Hadi Prayogo YHP, Anggini AW, Carolina A, Togu Manurung EG, Sari RK, Purwoko A, et al. In vitro and In silico Antibacterial Potency of Eucalyptus Leaf Oil from Hybrid Clones of E. grandis with E. urophylla and E. pellita. j.asep [Internet] 2025 [cité 2025 août 1];Available from: https://ojs.kmutnb.ac.th/index.php/ijst/article/view/7671

[8]. Hakim Haj Moussa, Benamara Sara, Hadia Benhalima, Fouzia Benaliouche, Ibtissem Sbartai, Hana Sbartai. Chemical characterization of Eucalyptus (Eucalyptus globulus) leaf essential oiland evaluation of its antifungal, antibacterial and antioxidant activities. Cell Mol Biol (Noisy-le-grand) 2025;70:1‑9.

[9]. Bejenaru LE, Segneanu AE, Bejenaru C, Biţă A, Tuţulescu F, Radu A, et al. Seasonal Variations in Chemical Composition and Antibacterial and Antioxidant Activities of Rosmarinus officinalis L. Essential Oil from Southwestern Romania. Applied Sciences 2025;15:681.

[10]. Belaid S, Chemlali I, Ben Rabeh S, Chamali S, Ben Romdhane C, Tlili N, et al. Essential oils, chemical composition, and biological activities of Eucalyptus oleosa F. Muell. : A review. J. OASIS AGRIC. SUSTAIN. DEV 2023;5:24‑33.

[11]. Banafa SH, Alasbahi RH. CHEMICAL COMPOSITION, EVALUATION OF RADICAL SCAVENGING AND ANTIBACTERIAL ACTIVITIES OF ESSENTIAL OIL LEAVES OF MENTHA LONGIFOLIA (L.) HUDSON SUBSP. SCHIMPERI (BRIQ.) BRIQ. CULTIVATED IN ADEN GOVERNMENT, YEMEN. Electron. J. Univ. Aden Basic Appl. Sci. 2024;5:494‑506.

[12]. Shyaula SL, Ghimire M, Maharjan S, Gurung K. Optimisation of Essential Oil Extraction of Lindera neesiana From Supercritical Carbon Dioxide Fluid and Comparison to Steam and Simultaneous Distillation Extraction. Flavour & Fragrance J 2025;40:267‑77.

[13]. Nguyen KV, Nguyen LKT, Le NT, Ho DV, Heinämäki J, Raal A, et al. Sustainable Extraction of the Tetrahydropalmatine Alkaloid from Stephania rotunda Lour. Tubers Using Eco-Friendly Solvent Systems. ACS Omega 2025;10:40313‑23.

[14]. Ionescu AD, Voicu G, Constantin GA, Ipate G, Ștefan EM, Begea M, et al. RESEARCH ON THE INFLUENCE OF GRINDING TIME ON THE PARTICLE SIZE DISTRIBUTION WHEN OBTAINING ROSEHIPS POWDER. INMATEH 2025;923‑33.

[15]. Muhammad Qasim Raza, Muhammad Sufyan, Anum Riaz, Laiba, Najam Ul Lail, Syeda Rameen Fatima, et al. IMPRESSIVE BENEFITS OF EUCALYPTUS LEAVES. Kashf J. Multidiscip. Res. 2024;1:25‑39.

[16]. Aabouch F, Annemer S, Satrani B, Ettaleb I, Kara M, Ghanmi M, et al. Assessing the Optimal Antibacterial Action of Lavandula stoechas L., Thymus zygis L., and Eucalyptus camaldulensis Dehnh Essential Oils. Life 2024;14:1424.

[17]. Liliya Naui, Abdelhamid Khaldi. Optimization of drying conditions to maximize essential oil yield of Crithmum maritimum L: Comparative analysis of methods and temperatures. World J. Adv. Res. Rev. 2025;25:1176‑81.

[18]. Loumouamou AN, Silou Th, Mapola G, Chalchat JC, Figuérédo G. Yield and Composition of Essential Oils From Eucalyptus citriodora x Eucalyptus torelliana , a Hybrid Species Growing in Congo-Brazzaville. Journal of Essential Oil Research 2009;21:295‑9.

[19]. Hedayati S, Tarahi M, Madani A, Mazloomi SM, Hashempur MH. Towards a Greener Future: Sustainable Innovations in the Extraction of Lavender (Lavandula spp.) Essential Oil. Foods 2025;14:100.

[20]. Alfian Z, Marpaung H, Taufik M, Lenny S, Andriayani, Samosir SJ. GC-MS Analysis of Chemical Contents and Physical Properties of Essential Oil of Eucalyptus grandis from PT. Toba Pulp Lestari. Asian J. Chem. 2019;31:2319‑22.

[21]. Tegegn G, Melaku Y, Aliye M, Abebe A, Degu S, Eswaramoorthy R, et al. Essential oil composition, in vitro antidiabetic, cytotoxicity, antimicrobial, antioxidant activity, and in silico molecular modeling analysis of secondary metabolites from Justicia schimperiana. Zeitschrift für Naturforschung C 2025;80:493‑516.

[22]. Afroz Shoily MstS, Islam MdE, Rasel NM, Parvin S, Barmon J, Hasan Aqib A, et al. Unveiling the biological activities of Heliotropium indicum L. plant extracts: anti-inflammatory activities, GC–MS analysis, and in-silico molecular docking. Sci Rep 2025;15:3285.

[23]. Elaissi A, Medini H, Khouja ML, Simmonds M, Lynen F, Farhat F, et al. Variation in Volatile Leaf Oils of Five Eucalyptus Species Harvested from Jbel Abderrahman Arboreta (Tunisia). Chemistry & Biodiversity 2011;8:352‑61.

[24]. Mohammadhosseini M, Kianasab MR, Nekoei M. Screening of the GC/MS profiles of the hydrodistilled essential oils and volatile components from the aerial parts of Datura stramonium L. Natural Product Research 2024;1‑5.

[25]. Xu Z, Zhu J, Gao P, Zhu X, Zhang Y, Liu X. Essential Oil From Tetrapanax papyrifer (Hook.) K. Koch: Chemical Composition, Antioxidant Activity, α‐Glucosidase Inhibitory Effect Integrating Molecular Docking Analysis. Chemistry & Biodiversity 2025;22:e202402533.

[26]. Belaid S, Gonzalez‐Coloma A, Andres MF, Elfalleh W, Idoudi S, Romdhane M, et al. Exploring Chemical Profiles, Antifeedant, Nematicidal, and Phytotoxic Potentials of Seven Essential Oils From Eucalyptus Species. Chemistry & Biodiversity 2025;22:e202402960.

[27]. Bulama Modu M, Yiğit Hanoğlu D, Hanoğlu A, Alkaş FB, Başer KHC, Özkum Yavuz D. Multivariate Statistical Analyses of the Temporal Variation in the Chemical Composition of the Essential Oil of Eucalyptus torquata in Cyprus. Molecules 2025;30:332.

[28]. Wang Y, Ma G, Huang X, Li X, Shao F. Comparison of Chemical Composition and Antimicrobial Activity of Dalbergia odorifera Essential Oils Obtained by Hydrodistillation and Supercritical Carbon Dioxide Extraction Methods. Natural Product Communications 2025;20:1934578X251313966.

[29]. El Orche A, El Mrabet A, Said AAH, Mousannif S, Elhamdaoui O, Ansari SA, et al. Integration of FTIR Spectroscopy, Volatile Compound Profiling, and Chemometric Techniques for Advanced Geographical and Varietal Analysis of Moroccan Eucalyptus Essential Oils. Sensors 2024;24:7337.

[30]. Ferraz TM, De Oliveira Maia Júnior S, De Souza GAR, Baroni DF, Rodrigues WP, De Sousa EF, et al. Clonal differences in ecophysiological responses to imposed drought in selected Eucalyptus grandis × E. urophylla hybrids. Tree Physiology 2025;45:tpae160.

[31]. Hindi S. 1, 8- Cineole Extracted from Eucalypt Ecotypes’ Leaves: I. A Novel Mi-2 crowave-Assisted Steam Distillation Method (MASD); Its Uploading 3 into Natural Polymeric Encapsules for Pest Control [Internet]. 2025 [cité 2025 oct 15];Available from: https://www.preprints.org/manuscript/202503.1324/v1

[32]. Dogara AM, Al-Zahrani AA, Bradosty SW, Hamad SW, Bapir SH, Anwar TK. Antioxidant, α-glucosidase, antimicrobial activities chemical composition and in silico analysis of eucalyptus camaldulensis dehnh. AIMSBPOA 2024;11:255‑80.

[33]. Saleem M, Shaheen MA, Qadir R, Saba I, Rehman MMU, Paracha RN, et al. Variations in the composition and biochemical attributes of Eucalyptus camaldulensis L. leaves essential oil as function of drying methods. Journal of Essential Oil Bearing Plants 2023;26:1266‑77.

[34]. Rajčević N, Dodoš T, Vujisić L, Novaković J, Janaćković P, Marin PD. Essential Oil Variability of Juniperus Oxycedrus s .l. Taxa in Southeast Europe–A Chemophenetic Approach. Chemistry & Biodiversity 2025;22:e202403110.

[35]. Jawad.kadhim K, Hanawi MJ, Abbood HS, Hammadi HA. Antifungal Properties of Eucalyptus Camaldulensis Essential Oil Against Aspergillus Spp. SEEJPH 2024;34‑44.

[36]. Isyaka MS, Sudi MJ, Anyam VJ, Labaran HS, Muhammad AA. ANTIMICROBIAL ASSAY OF ESSENTIAL OIL FROM Eucalyptus globulus LEAF. JCSN [Internet] 2024 [cité 2025 sept 18];49. Available from: https://journals.chemsociety.org.ng/index.php/jcsn/article/view/1025

[37]. Tanasă F, Nechifor M, Teacă CA. Essential Oils as Alternative Green Broad-Spectrum Biocides. Plants 2024;13:3442.