Applied Chemical Engineering

Methanolic extraction of three plants and their biological activity against different pathogenic bacteria

Haider Abbas AbdulRedha

Department of Biology, Faculty of Science, University of Kufa, Kufa, Najaf, 540011, Iraq

Nawras Alwan Al- Barqawi

Department of Biology, Faculty of Science, University of Kufa, Kufa, Najaf, 540011, Iraq

Najla Saleh Mahdi

Department of Biology, Faculty of Science, University of Kufa, Kufa, Najaf, 540011, Iraq

Ahmed A. A. Madhloom

Department of Biology, Faculty of Science, University of Kufa, Kufa, Najaf, 540011, Iraq

Zahraa Yosif Motaweq

Department of Biology, Faculty of Science, University of Kufa, Kufa, Najaf, 540011, Iraq

DOI: https://doi.org/10.59429/ace.v9i1.5815

Keywords: methanol extract; Punica; Citrullus colocynthis; Cordia myxa; biological activity; antioxidant

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Abstract

The increased new and remerging infectious diseases, need to discovery and urgent of new microbial compounds having diverse chemical structures and novel mechanism have also been. The study focused on determining the antibacterial properties of  pomegranate peels (Punica granatum), Cordia myxa fruits, and Citrullus colosynthis fruits, against pathogenic bacteria E.coli, Pseudomonas aeruginosa as Gram-negative and Staphylococcus aureus as Gram-negative. Also the antioxidant as free radical scavenge to the plants   The plant parts were collected, shade dried and powdered and subjected to extraction by methanol maceration as solvents. The extracts were used to determine the antibacterial activity by agar well diffusion method and MIC assay. The results showed that the plant extracts inhibited the growth of both Gram-positive and negative bacteria, Gram-negative bacteria were more sensitive. The inhibition zone (in millimeters) was greater for Gram-negative bacteria (17 mm) than for Gram-positive bacteria (0 mm) with Cordia myxa. Punica peel and Citrullus colocynthis fruits extracts exhibited high biological activity against all types of bacteria at concentrations of 0.1, 0.5, and 1 mg/mL, respectively. Cordia myxa had the highest percentage of free radical scavenging, followed by Punica and then Citrullus colocynthis. On the basis of the Data obtained it is clear that plant extracts can be used as effective herbal cure against human pathogens.

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