Applied Chemical Engineering

Green synthesis of silver nanoparticles (CC-Ag NPs) utilizing the cymbopogon citratus extract and studying the antibacterial activity

Sahar A. Mamoori

Department of Soil Science and Water Resource, College of Agriculture, Al-Qasim Green University, Al-Qasim District 964, Babylon 51013, Iraq

Ameer S. Muttaleb

Department of Soil Science and Water Resource, College of Agriculture, Al-Qasim Green University, Al-Qasim District 964, Babylon 51013, Iraq

Issa Farhan D.

Department of Optics Technologies, College of Health & Medical Techniques, Al-Mustaqbal University, Babylon, 6163, Iraq

Ehab K. Obaid

Department of Soil Science and Water Resource, College of Agriculture, Al-Qasim Green University, Al-Qasim District 964, Babylon 51013, Iraq

Ali Jabbar Radhi

College of Pharmacy, University of Al-Kafeel, Najaf, 54001, Iraq

Amjed Mirza Oda

Department of Science, College of Basic Education, University of Babylon, 51002, Iraq

DOI: https://doi.org/10.59429/ace.v9i1.5847

Keywords: silver nanoparticles; antibacterial; cymbopogon citratus; AFM; XRD

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Abstract

CC-Ag NPs (Silver nanoparticles) were synthesized from the leaf extract of Cymbopogon citratus. The biosynthesized silver nanoparticles were characterized by different analytical techniques. A characteristic absorption peak at 432 nm was observed which confirmed the surface plasmon resonance in silver nanoparticles (AgNPs). FTIR analysis showed the presence of bioactive compounds that take part in reduction and stabilization processes. The results of XRD analysis showed a crystalline phase formation only corresponding to silver nanoparticles. AFM and SEM analyses revealed that most of the particles are spherical with an average particle size of about 40 nm. The biosynthesized silver nanoparticles showed strong antibacterial effects against different pathogenic bacteria that covered both Gram-positive and Gram-negative strains. These included Proteus mirabilis, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella enteritidis. Among all these tested organisms, the maximum zone of inhibition was observed against Vibrio parahaemolyticus with a diameter of 34.67mm. The concentration of the synthesized AgNPs solution used in these experiments was 1 mM.

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