Applied Chemical Engineering

Ensuring Drug Quality for Public Health: A Comparative HPLC and UV-Vis Study of Iraqi Market Furosemide Analysis Aligned with SDG 3

Salam Mohammed Nasser

Department of Chemistry, College of Science, University of Babylon, Babel, Iraq.

Rusl Mahdi Obaid

Department of Chemistry, College of Science, University of Babylon, Babel, Iraq.

Ahmed Ali Alkarimi

sci.ahmed.ali@uobabylon.edu.iq

DOI: https://doi.org/10.59429/ace.v9i1.5860

Keywords: SDG-3; Furosemide; HPLC; UV-Vis

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Abstract

Reliable furosemide quantification in pharmaceutical formulations is critical to ensuring drug quality and regulatory compliance. The purpose of this study was to develop and compare spectrophotometric and high-performance liquid chromatography (HPLC) methods for determining furosemide in pharmaceutical dosage forms found in local pharmacies. Standard solutions of furosemide in the concentration range of 2-30 ppm were prepared to create calibration curves using UV-visible spectrophotometry and HPLC methods. Under optimum experimental conditions, the proposed methods' analytical performance was assessed in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), molar absorptivity, and Sandell's sensitivity. The spectrophotometric method was linear, with a correlation coefficient (R²) of 0.999 and an absorption coefficient of 0.0596 at a wavelength of 275 nm. The calculated LOD and LOQ values were 0.721 ppm and 1.069 ppm. A C18 column with a mobile phase of water, ethanol, and acetonitrile was used for chromatographic analysis under controlled temperature conditions. The results of both analytical methods indicated that the measured drug content in all tested pharmaceutical samples was consistent with the labeled amounts. The developed methods demonstrated satisfactory accuracy, sensitivity, and applicability for routine quality control of furosemide in pharmaceutical formulations, resulting in dependable and cost-effective analytical approaches for laboratory use.

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