Applied Chemical Engineering

Spectral and Non-Linear Optical Properties Investigation of Mixtures of (Fluorescein, Eosin, and Rhodamine B) Organic Laser Dyes

Alaa Ali Diwan Shamkhi

Department of Physics, College of Education for Pure Science, University of Babylon, Babylon 51002, Iraq

Ban A. Naser

Department of Physics, College of Science, University of Babylon, Babylon 51002, Iraq

Nihal Abdullah AbdulWahhab

Department of Physics, College of Science, University of Babylon, Babylon 51002, Iraq

DOI: https://doi.org/10.59429/ace.v9i2.5912

Keywords: Eosin dye, quantum efficiency, photonic applications, nonlinear refractive index, self-focusing

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Abstract

This study investigates the spectral and nonlinear optical characteristics of a new mixture of three organic laser dyes : (Fluorescein Orange, Eosin Yellow, and Rhodamine B) dissolved in chloroform solvent at varying concentrations (2×10-5, 4×10-5, 6×10-5, and 8×10-5) M. A diode-pumped solid-state laser with a power of 84mW and a 457nm wavelength has been used for nonlinear measurements using the Z-Scan method.   With a UV-VIS spectrophotometer, the transmission and absorption spectra of each sample were measured.  All of the samples' closed-aperture Z-scans confirmed self-defocusing behavior, and the open-aperture Z-scan confirmed two-photon absorption. According to the findings, the nonlinear absorption coefficient and nonlinear refractive index increased with concentrations. The fluorescence spectra of the dye combination at a concentration of (8×10⁻⁵ M) were the most intense among all tested concentrations .The findings showed that there was a clear link between concentration and absorption intensity :when concentration went up, absorbance went up as well. The research also found that larger concentrations led to a lower quantum efficiency and a longer fluorescence lifespan. These results indicate that the dye combination has favorable properties, positioning it as a good candidate for use in photonic and nonlinear optical devices.

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References

[1]. Sarah M. Khalil , Afrah M. Al Hussainey2, Alhak A. Hassan, Ban A. Naser, and Menaa J.Alwan " Optical limiting characteristics of organic laser dye ", AIP Conference Proceedings , 2776, 070014,( 2023).

[2]. Kadhum, S.H., Al Hussainey, A.M., Naser, B.A., Mahdi, R.S., Abbas, T.M. "Linear optical properties of organic laser dye doped with PMMA polymer", NeuroQuantology, 20(3): 364-367,( 2022).

[3]. Amit Nag, Debabrata Goswami,"Solvent Effect on two-Photon Absorption and Fluorescence of Rhodamine Dyes",National Library of Medicine, 188-197, (2009).

[4]. Al-Aaraji, N.A.A, Abdulazeez O. Mousa, Ban A. Naser " Spectral and Linear Optical Characterization of Rhodamine B an Fluorescein Sodium Organic Laser Dyes Mixture Solutions" Iraqi Journal of Science, Special Issue, pp: 69-74 ,(2019).

[5]. Al-Hussainey, A.M., Al-Shafie, T.M.A. , "Study the optical and spectral properties of organic dye as an effective medium in dye lasers". Journal of Global Pharma Technology, 10(5): 304-311. (2009).

[6]. Alnayli, R.S., Shanon, Z.S., and Hadi, A.S., "Study the linear and nonlinear optical properties for laser dye Rhodamine B", Journal of Physics: Conference Series, 1234(1): 012022, (2019).

[7]. Kadhum, S.H., Abbas, T.M. , "Study of fluorescence energy transfer for mixture of two laser organic dyes. Journal of Advanced Research in Dynamical and Control Systems", 12(7): 774-781,(2020).

[8]. Mohammad, R.K., Aboud, L.H., Jassim, A.H. ," Study of molecular electronic energy levels of malachite green dye". AIP Conference Proceedings, 2144(1): 030022,(2019).

[9]. Nooralhuda J.Abdulkadhim, Ban A. Naser and Abdulazeez Q.Mousa, "Optical Limiting Properties of Liquid Crystals Doped by CuCl 2 Nanoparticles", Journal of Engineering and Applied Sciences 13(22):9553-9557,(2018).

[10]. P. Biswas and Y.Chang, " Nanopartical and the Environment", Journal of Air and Waste Management Association, Taylor and Francis Group, 55,6,708-746,(2005).

[11]. Ali, F.H., Naser, B.A. ,"Optical properties for new mixture of two organic laser dyes", Journal of Advanced Research in Dynamical and Control Systems, 12(7): 768-773, (2020).

[12]. Fiedler S.E., and Achim Hese, A.A. Ruth,"Incoherent broad-band cavity-enhanced absorption spectroscopy", (IBBCEAS), (2003).

[13]. Khalil S.M., Hussainey A. M.A., Hassan, A.A, Ban A. Naser, and Alwan M.J, "Optical limiting characteristics of organic laser dye". AIP Conferenc Proceedings, 2776(1): 070014, (2023).

[14]. Fahdel Odah, J., Jaddoa, M.F., Mohamed, A.N, " Effect of adding MgO and NiO nano-oxides on the spectroscopic properties of Nile Blue laser dye". Journal of Optics, 53(1): 493-499, (2024).

[15]. Nooraldeen, A.Y., Palanichant, M., Palanisamy, P.K. , "Influence of solvents polarity on NLO properties of fluorone dye". International Journal of Nonlinear Science, 7(3): 290-300, (2009).

[16]. Khudhair, O.S., Mousa, A.O., Rassol, S.R., "The effect of mixing two organic dyes on the optical linear properties". Natural Volatiles & Essential Oils, 8(4): 8128-8138, (2021).

[17]. Sowmya Maiya, Glanish Jude Martis, Nitinkumar S.Shetty and Santosh L.Gaonkar,"Orgain fluorescent compounds: review of synthetic strategies and emerging application", Discover Applied Science, 7:1322,(2025).

[18]. Ashieigh Smith, Selin Ergulen , Meredith M.Ogle and Angel A.Marti," Fluorescent surfactants from common dyes – Rhodamine B and Eosin Y", Pure and Applied Chemistry,pac-2019-0219,(2018).

[19]. Sigma-Aldrich, “Sigma-Aldrich Homepage,” Sigma-Aldrich, 2025. [Online]. Available: https://www.sigmaaldrich.com/IQ/en. [Accessed: Aug. 18, 2025].

[20]. Ule, E. , "Measurement of the nonlinear refractive index by Z-scan technique. University of Ljubljana", Slovenia, (2015).

[21]. Aboud, L.H., Mahdi, Z.F., Al-Zahra, W.J.A. ," Linear and nonlinear optical properties of the dye laser (acridine dye)". Academic Research International, 5(4), (2014).

[22]. Kumaresan, S., Ahamed, M.B. ,"Nonlinear optical properties of pyronin b dye by Z-scan technique using q-switched pulsed Nd: YAG laser" . International Journal of ChemTech Research. CODEN, 8(3): 1163- 1167, (2015).

[23]. F.Mahasin and E. Mohi , "FTIR spectrum of laser dye fluorescein doped polymer PMMA films " , Research and Reviews in Polymer 3(3), 102-106, (2012).

[24]. Ali.A and K. Hussaain," Spectroscopic investigation of Rhodamine-B thin film prepared by PLD technique", Iraqi Journal of Physics, University of Baghdad, Vol.17 NO.40(2019).

[25]. Ayad F.AL-Kaim, Abass S.AL-Watefi and Zeyad Umraan,"Study the adsorption of eosin dye by modified clay with urea-formaldehyde polymer",National Journal of Chemistry, Volume 29,87-108,(2008).

[26]. AL-Juboury, I.A.M., Ali, S.M.H., Abdulkhudher, B.A.,Mohammed, M. R. "Linear optical properties of (NB Dye-PVP/AuNps) nanocomposite. Azerbaijan Pharmaceutical and Pharmacotherapy Journal" , 22: 205- 209,(2023).

[27]. Safa Ahmed Jabbar , Ban A. Naser and Samera A. Mahdi1, (2024). "Non-Linear and Linear Optical Properties of an Organic Laser Dye Mixture", pp. 401-407. (2024).

[28]. Albouchi, F., Abdelmajid, J. ," Photothermal investigations of conductive and optical properties of liquids in the near infrared. Instrumentation Mesure Métrologie", 20(1): 49-56, (2021) .

[29]. Sayed M. Derayea and Dalia M. Nagy, "Application of A Xanthene Dye, Eosin Y, As Spectroscopic Probe In Chemical And Pharmaceutical Analysis", A review , (2018).

[30]. Albouchi, F., Abdelmajid, J. ," Photothermal investigations of conductive and optical properties of liquids in the near infrared. Instrumentation Mesure Métrologie", 20(1): 49-56, (2021) .

[31]. B.Bhushan,"Handbook of Nanotechnology",3rd. ed., ISBN: 978-3-642-02524-2, Springer-Verlag Berlin Heidelberg,(2010).

[32]. Zhi-Xiang Han, Xiao-Bing Zhang ,Zhuo Li ,Yi-Jun Gong ,Xiang-Yang ,Chun-Mei He and Ru-Qin Yu," Efficient Fluorescence Resonance Energy Transfer-Based Ratiometric Fluorescent Cellular Imaging Probe for Zn2+ Using a Rhodamine Spirolactam as a Trigge", Analytical Chemistry,3108-3113,(2010).

[33]. Ban R. Saleh, Ban A. Naser and Zahraa N. Salman, "Non-Linear Optical Properties for Thin Films of Fluorescein Organic Laser Dyes Doped with Polyvinyl Alcohol Polymer and Al2O3 Nanoparticles", Eng. Proc,59, 141, (2023).

[34]. David Barbalas ,Ralph Romero, Dipanjan Chaudhuri, Fahad Mahmood, and N.P.Armitage,"Energy Relaxation and Dynamics in the Correlated Metal Sr2RuO4 via Terahertz Two-Dimensional Coherent Spectroscopy" APS Physical Review Journals,134,036501,(2025).

[35]. Sinha, S., Ray, A., "Dasgupta, K.Solvent dependent nonlinear refraction in organic dye solution", Journal of Applied Physics, 87(7): 3222-3226, (2000).

[36]. Saleh, B.R., Naser, B.A., Salman, Z.N. , "Non- Linear Optical Properties for Thin Films of Fluorescein Organic Laser Dyes Doped with Polyvinyl Alcohol Polymer and Al2O3 Nanoparticles". Engineering Proceedings, 59(1): 141, (2024).