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2024-09-13
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Copyright (c) 2024 Yasser Fakri Mustafa
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How to Cite
Coumarins from toxic phenol: An algorithm of their synthesis and assessment as biosafe, wide-spectrum, potent antimicrobial prospects
Yasser Fakri Mustafa
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, Iraq
DOI: https://doi.org/10.59429/ace.v7i3.5527
Abstract
The existential war between pathogens and humans has heavily intensified during the last few decades. The former war side has been strengthened by developing various mechanisms of resistance to the currently-in-use antimicrobial drugs. To overcome the consequences of this development, it becomes an urgent global request to explore new potent, wider-ranging, and biosafe prospects as antimicrobial medications. In response to this request, this work was designed to include three parts. In the first one, coumarin-based compounds were created using a toxic material named 2-methyl-3,5-dinitrophenol as a starting block. The Pechmann condensation reaction was conducted to convert this building block to the precursor, P-MDNP, which was esterified with various phenols to create MDNPU1–MDNPU10. The antimicrobial function was evaluated in the second study part using a broth microdilution approach and three standards, including ciprofloxacin, metronidazole, and nystatin. The studied pathogens were four-infectious bacterial aerobes, four-infectious bacterial anaerobes, and two-infectious fungi. Given the third study part, the biosafety of the synthesized compounds was quantified on the three healthy cellular species, two non-infectious aerobic bacteriomers, and human blood processed in the lab. The synthesized compounds showed strong, wide-ranging, and biosafe antimicrobial properties versus the pathogens examined, according to the outcomes. Moreover, the study showed that some of these compounds demonstrated anti-anaerobic bacterial activity that is superior to metronidazole. Furthermore, the study found a connection between the number and distribution of chlorides in the off-side aromatic rings, antimicrobial activity, and biosafety. Finally, it is determined that the health-damaging effects of the toxicant under study can be mitigated by grafting it into coumarin frameworks. These are potent, ascribed to MDNPU9, and have great levels of biosafety and wider-ranging antimicrobial efficacy. Furthermore, this approach offered the chance to turn the health-detrimental effects of the nitrophenols into potential benefits. Coumarin-4-acetic acid and MDNPU9 can be employed as a synthetic fragment and a bioactive scaffold, respectively, to accomplish this.
References
[1]. V. H. Parker. The effect of 3:5-dinitro-ortho-cresol on phosphocreatine and the adenosine phosphate compounds of rat tissues. Biochemical Journal. 1954;57(3):381–386.
[2]. Bidstrup PL, Payne DJH. Poisoning by Dinitro-Ortho-Cresol. BMJ. 1951;2(4722):16-19.
[3]. Mustafa YF, Zain Al-Abdeen SH, Khalil RR, Mohammed ET. Novel functionalized phenyl acetate derivatives of benzo [e]-bispyrone fused hybrids: Synthesis and biological activities. Results in Chemistry. 2023;5:100942.
[4]. Mustafa YF. Modern Developments in the Application and Function of Metal/Metal Oxide Nanocomposite–Based Antibacterial Agents. BioNanoScience. 2023;13:840-852.
[5]. Mustafa YF. Biocompatible chlorocoumarins from harmful chlorophenols, their synthesis and biomedicinal evaluation. Journal of Molecular Structure. 2024;1309:138193.
[6]. Zeki MN, Mustafa YF. Synthesis and evaluation of novel ring-conjugated coumarins as biosafe broad-spectrum antimicrobial candidates. Journal of Molecular Structure. 2024;1309:138192.
[7]. Jibroo RN, Mustafa YF, Al-Shakarchi W. Synthesis and evaluation of linearly fused thiadiazolocoumarins as prospects with broad-spectrum bioactivity. Results in Chemistry. 2024;7:101494.
[8]. Teijaro CN, Adhikari A, Shen B. Challenges and opportunities for natural product discovery, production, and engineering in native producers versus heterologous hosts. Journal of Industrial Microbiology and Biotechnology. 2019;46(3-4):433-444.
[9]. Younes AH, Mustafa YF. Unveiling the Biomedical Applications of Novel Coumarins Isolated From Capsicum Annuum L. Seeds by a Multivariate Extraction Technique. Chemistry and Biodiversity. 2024;21(6):e202400581.
[10]. Jasim SF, Mustafa YF. Synthesis and Antidiabetic Assessment of New Coumarin-Disubstituted Benzene Conjugates: An In Silico-In Virto Study. Journal of Medicinal and Chemical Sciences. 2022;5(6):887-899.
[11]. Mustafa YF, Oglah MK, Bashir MK, Mohammed ET, Khalil RR. Mutual prodrug of 5-ethynyluracil and 5-fluorouracil: Synthesis and pharmacokinetic profile. Clinical Schizophrenia and Related Psychoses. 2021;15(5):1-6.
[12]. Khalil RR, Mohammed ET, Mustafa YF. Evaluation of in vitro antioxidant and antidiabetic properties of Cydonia Oblonga seeds’ extracts. Journal of Medicinal and Chemical Sciences. 2022;5(6):1048-1058.
[13]. Kasim SM, Abdulaziz NT, Mustafa YF. Synthesis and biomedical activities of coumarins derived from natural phenolic acids. Journal of Medicinal and Chemical Sciences. 2022;5(4):546-560.
[14]. Akkol EK, Genç Y, Karpuz B, Sobarzo-Sánchez E, Capasso R. Coumarins and coumarin-related compounds in pharmacotherapy of cancer. Cancers. 2020;12(7):1959.
[15]. Kummerle AE. Coumarin compounds in medicinal chemistry: Some important examples from the last year. Current Topics in Medicinal Chemistry. 2018;18(5):124-148.
[16]. Jasim SF, Mustafa YF. A Review of Classical and Advanced Methodologies for Benzocoumarin Synthesis. Journal of Medicinal and Chemical Sciences. 2022;5(5):676-694.
[17]. Waheed SA, Mustafa YF. Benzocoumarin backbone is a multifunctional and affordable scaffold with a vast scope of biological activities. Journal of Medicinal and Chemical Sciences. 2022;5(5):703-721.
[18]. Mustafa YF, Bashir MK, Oglah MK. Original and innovative advances in the synthetic schemes of coumarin-based derivatives: A review. Systematic Reviews in Pharmacy. 2020;11(6):598-612.
[19]. ActaŠeršeň F, Lácová M. Antioxidant activity of some coumarins. Acta Facultatis Pharmaceuticae Universitatis Comenianae. 2015;2015(Suppl IX):41-45.
[20]. Mustafa YF. 4-Chloroskimmetine-based derivatives as potential anticancer and antibacterial prospects: Their synthesis and in vitro inspections. Results in Chemistry. 2024;7:101511.
[21]. Mohammed ET, Khalil RR, Mustafa YF. Phytochemical Analysis and Antimicrobial Evaluation of Quince Seeds’ Extracts. Journal of Medicinal and Chemical Sciences. 2022;5(6):968-979.
[22]. Yusufzai SK, Khan MS, Sulaiman O, Osman H, Lamjin DN. Molecular docking studies of coumarin hybrids as potential acetylcholinesterase, butyrylcholinesterase, monoamine oxidase A/B and β-amyloid inhibitors for Alzheimer’s disease. Chemistry Central Journal. 2018;12(1):128.
[23]. Kamel NN, Aly HF, Fouad GI, et al. Anti-Alzheimer activity of new coumarin-based derivatives targeting acetylcholinesterase inhibition. RSC Advances. 2023;13(27):18496-18510.
[24]. Mustafa YF. Combretastatin A4-based coumarins: synthesis, anticancer, oxidative stress-relieving, anti-inflammatory, biosafety, and in silico analysis. Chemical Papers. 2024;78:3705–3720.
[25]. Chamlagai D, Bora P, Bhatta A, et al. Donor-acceptor functionalized coumarin derivatives: Synthesis, fluorescence modulation, interaction with human serum albumin and acetylcholinesterase inhibition activity. Journal of Photochemistry and Photobiology A: Chemistry. 2024;447:115273.
[26]. Adsule P, Purandare D, Kulkarni S, Joshi R, Chabukswar A. Synthesis and Evaluation of Analgesic and Antioxidant Activity of 3-Phenyl Coumarin Derivatives. Asian Journal of Chemistry. 2023;35(9):2109-2114.
[27]. Aleru O, Barber MF. Battlefronts of evolutionary conflict between bacteria and animal hosts. Coers J, ed. PLOS Pathogens. 2020;16(9):e1008797.
[28]. Uddin TM, Chakraborty AJ, Khusro A, et al. Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects. Journal of Infection and Public Health. 2021;14(12):1750-1766.
[29]. Peterson E, Kaur P. Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens. Frontiers in Microbiology. 2018;9.
[30]. Impey RE, Hawkins DA, Sutton JM, Soares da Costa TP. Overcoming Intrinsic and Acquired Resistance Mechanisms Associated with the Cell Wall of Gram-Negative Bacteria. Antibiotics. 2020;9(9):623.
[31]. Sultan I, Rahman S, Jan AT, Siddiqui MT, Mondal AH, Haq QMR. Antibiotics, Resistome and Resistance Mechanisms: A Bacterial Perspective. Frontiers in Microbiology. 2018;9.
[32]. Jebir RM, Mustafa YF. Natural Products Catalog of Allsweet Watermelon Seeds and Evaluation of Their Novel Coumarins as Antimicrobial Candidates. Journal of Medicinal and Chemical Sciences. 2022;5(5):831-847.
[33]. Mustafa YF. Coumarins derived from natural methoxystilbene as oxidative stress-related disease alleviators: Synthesis and in vitro-in silico study. Journal of Molecular Structure. 2024;1302:137471.
[34]. Mustafa YF. Triple coumarin-based 5-fluorouracil prodrugs, their synthesis, characterization, and release kinetics. Journal of Molecular Structure. 2024;1301:137415.
[35]. Jebir RM, Mustafa YF. Novel coumarins isolated from the seeds of Citrullus lanatus as potential antimicrobial agents. Eurasian Chemical Communications. 2022;4(8):692-708.
[36]. Mustafa YF. Synthesis, in silico analysis, and biomedical effects of coumarins derived from resveratrol. Phytomedicine Plus. 2024;3(4):100501.
[37]. Waheed SA, Mustafaa YF. Novel naphthalene-derived coumarin composites: synthesis, antibacterial, and antifungal activity assessments. Eurasian Chemical Communications. 2022;4(8):709-724.
[38]. Mustafa YF. Coumarins from carcinogenic phenol: synthesis, characterization, in silico, biosafety, anticancer, antioxidant, and anti-inflammatory assessments. Chemical Papers. 2024;78:493-504.
[39]. Mustafa YF, Ismael RN, Jebir RM. Natural coumarins from two cultivars of watermelon seeds as biosafe anticancer agents, an algorithm for their isolation and evaluation. Journal of Molecular Structure. 2024;1295(P1):136644.
[40]. Faisal S, Shah SA, Shah S, et al. In Vitro Biomedical and Photo-Catalytic Application of Bio-Inspired Zingiber officinale Mediated Silver Nanoparticles. Journal of biomedical nanotechnology. 2020;16(4):492-504.
[41]. Weber M, Steinle H, Golombek S, et al. Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility. Frontiers in Bioengineering and Biotechnology. 2018;6.
[42]. Blinova A, Blinov A, Kravtsov A, et al. Synthesis, Characterization and Potential Antimicrobial Activity of Selenium Nanoparticles Stabilized with Cetyltrimethylammonium Chloride. Nanomaterials. 2023;13(24):3128.
[43]. Mustafa YF. Emerging trends and future opportunities for coumarin-heterocycle conjugates as antibacterial agents. Results in Chemistry. 2023;6:101151.
[44]. Dubovoy V, Nawrocki S, Verma G, et al. Synthesis, Characterization, and Investigation of the Antimicrobial Activity of Cetylpyridinium Tetrachlorozincate. ACS Omega. 2020;5(18):10359-10365.
[45]. Mustafa YF, Bashir MK, Oglah MK. Original and innovative advances in the synthetic schemes of coumarin-based derivatives: A review. Systematic Reviews in Pharmacy. 2020;11(6):598-612.
[46]. Zeki NM, Mustafa YF. Novel heterocyclic coumarin annulates: synthesis and figuring their roles in biomedicine, bench-to-bedside investigation. Chemical Papers. 2024;78:4935-4951.
[47]. Nejres AM, Ali HK, Behnam SP, Mustafa YF. Potential effect of ammonium chloride on the optical physical properties of polyvinyl alcohol. Systematic Reviews in Pharmacy. 2020;11(6):726-732.
[48]. Raya I, Chupradit S, Kadhim MM, et al. Role of Compositional Changes on Thermal, Magnetic and Mechanical Properties of Fe-P-C-Based Amorphous Alloys. Chinese Physics B. 2022;31(1):016401.
[49]. Mustafaa YF. New Coumarin-Metronidazole Composites: Synthesis, Biocompatibility, and Anti-anaerobic Bacterial Activity. Russian Journal of Bioorganic Chemistry. 2024;50(1):201-210.
[50]. Jasim SF, Mustafa YF. Synthesis, ADME Study, and antimicrobial evaluation of novel naphthalene-based derivatives. Journal of Medicinal and Chemical Sciences. 2022;5(5):793-807.
[51]. Rani VE, Reddy PR. Synthesis and Antimicrobial Activity of New Pyridine Containing Substituted Phenyl Azetidine-2-One Derivatives. Open Journal of Medicinal Chemistry. 2018;08(02):22-29.
[52]. Mustafa YF. Harmful Free Radicals in Aging: A Narrative Review of Their Detrimental Effects on Health. Indian Journal of Clinical Biochemistry. 2024;39(2):154-167.
[53]. Betancur M, López J, Salazar F. Antimicrobial activity of compounds from hop (Humulus lupulus L.) following supercritical fluid extraction: An overview. Chilean journal of agricultural research. 2023;83(4):499-509.
[54]. Firoozeh AZ, Bokov DO, Salahdin OD, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF7 cancer cells. Rendiconti Lincei Scienze Fisiche e Naturali. 2022;33:441-447.
[55]. Gziut T, Thomas SHL. International trends in systemic human exposures to 2,4 dinitrophenol reported to poisons centres. Clinical Toxicology. 2022;60(5):628-631.
[56]. Zhao X hong, Jiang J kun, Lu Y qiang. Evaluation of efficacy of resin hemoperfusion in patients with acute 2,4-dinitrophenol poisoning by dynamic monitoring of plasma toxin concentration. Journal of Zhejiang University: Science B. 2015;16(8):720-726.
[57]. Jung M, Lee SJ, Yoo SH, Kim H. Death from 2,4-Dinitrophenol Poisoning: An Autopsy Case. Korean Journal of Legal Medicine. 2020;44(3):140-142.
[58]. McGillis ES, Olives TD, Cole JB. Reply: Matching minute ventilation in the hypermetabolic state of dinitrophenol poisoning. Annals of the American Thoracic Society. 2020;17(11):1498.
[59]. Fang W-Y, Ravindar L, Rakesh KP, et al. Synthetic approaches and pharmaceutical applications of chloro-containing molecules for drug discovery: A critical review. European Journal of Medicinal Chemistry. 2019;173:117-153.
[60]. Zeki NM, Mustafa YF. 6,7-Coumarin-heterocyclic hybrids: A comprehensive review of their natural sources, synthetic approaches, and bioactivity. Journal of Molecular Structure. 2024;1303:137601.
[61]. Ramalingam A, Mustafa N, Chng WJ, Medimagh M, Sambandam S, Issaoui N. 3-Chloro-3-methyl-2,6-diarylpiperidin-4-ones as Anti-Cancer Agents: Synthesis, Biological Evaluation, Molecular Docking, and In Silico ADMET Prediction. Biomolecules. 2022;12(8):1093.
[62]. Atia YA, Bokov DO, Zinnatullovich KR, et al. The role of amino acid functionalization for improvement of adsorption Thioguanine anticancer drugs on the boron nitride nanotubes for drug delivery. Materials Chemistry and Physics. 2022;278:125664.
[63]. Faisal S, Abdullah, Jan H, et al. Bio-catalytic activity of novel mentha arvensis intervened biocompatible magnesium oxide nanomaterials. Catalysts. 2021;11(7):1-18.
[64]. Nasar MQ, Khalil AT, Ali M, Shah M, Ayaz M, Shinwari ZK. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials. Medicana. 2019;55:369-385.